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This corrects the article “Analysis of Tau Protein Expression in Predicting Pathological Complete Response to Neoadjuvant Chemotherapy in Different Molecular Subtypes of Breast Cancer” in volume 23 on page 47.This article was initially published on the Journal of Breast Cancer with a misspelled the abbreviation in figure 3. The abbreviation ‘HP’ should be corrected as ‘HR’.
ABSTRACT
PURPOSE: Tau is a microtubule-associated protein that can be found in both normal and abnormal breast cells. Whether the expression of Tau protein can predict the response to neoadjuvant chemotherapy (NACT) is still unclear. In this study, we assessed the role of Tau protein expression in predicting a pathological complete response (pCR) to NACT for different subtypes of breast cancer.METHODS: Four hundred and sixty-eight eligible patients were retrospectively recruited in our study. The relationship between clinicopathologic factors, including Tau protein expression, and pCR in different subtypes was evaluated using logistic regression analysis. Correlation between Tau and disease-free survival (DFS) and overall survival (OS) was performed using Kaplan–Meier analysis.RESULTS: The expression of Tau protein was negatively correlated with pCR, especially in triple-negative breast cancer (TNBC). No significant difference was observed in the luminal human epidermal growth factor receptor-2 (HER2)-negative subtype and HER2-positive subtype. Patients with pCR were associated with better DFS and OS (p < 0.05). However, Tau protein expression had no association with either DFS or OS (p > 0.05).CONCLUSION: Tau protein expression can predict pCR before NACT in TNBC, but there was no correlation between Tau expression and DFS or OS.
Subject(s)
Humans , Breast Neoplasms , Breast , Disease-Free Survival , Drug Therapy , Epidermal Growth Factor , Logistic Models , Neoadjuvant Therapy , Phenobarbital , Polymerase Chain Reaction , Retrospective Studies , tau Proteins , Triple Negative Breast NeoplasmsABSTRACT
BACKGROUND:Now it has cooperation and facilitative effete between myogenic regulatory factors through a long time study. So, gene therapy of double genes of Myod1 and Myog can obtain better effect, and can provide a new way for preventing denervated skeletal muscle atrophy. OBJECTIVE:To construct eukaryotic co-expression vector carrying Myod1 and Myog genes. METHODS:Ful-length Myod1 gene and Myog gene cDNA were amplified by reverse transcription PCR, and then inserted into pVAX1 vector after digested to establish the recombined Myod1 and Myog eukaryotic co-expression vector pVAX1-Myod1-IRES2-Myog-IRES2-EGFP, and then identified with gene sequencing. The in vitro cultured 3T3 cel s were transfected with pVAX1-Myod1-IRES2-Myog-IRES2-EGFP, and the expressions of Myod1 and Myog genes in the 3T3 cel s were detected with western blot assay in order to identify whether the 3T3 cel s could express the target protein correctly. RESULTS AND CONCLUSION:The sequencing results showed that the sequence length and base sequence of Myod1 and Myog cDNA in eukaryotic co-expression vector pVAX1-Myod1-IRES2-Myog-IRES2-EGFP were identical with the reported sequences. Myod1 and Myog protein band expressions were detected in 3T3 cel s by western blot after transient transfection. The pVAX1-Myod1-IRES2-Myog-IRES2-EGFP, a eukaryotic co-expression vector of Myod1 gene and Myog gene is successful y constructed.
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Objective To investigate the significance of Qmax and residual urine in evaluation of bladder function in the patients with benign prostatic hyperplasia (BPH).Methods The clinical data of 61 patients with BPH and 20 healthy persons in control group were evaluated.Bladder function,uroflowmetry and ultrasonic residual urine measurement were performed in the 2 groups.The correlation between Q max and residual urine in BPH group was investigated.Results There was significant difference in Qmax between the BPH group and control group (8 vs.21 ml/s,u=-6.090,P=0.007).There was significant difference in residual urine between the 2 groups (60 vs.9 ml,u =-6.718,P=0.005).And there was a negtive correlation between residual urine and Qmax in BPH group (r=-0.366,P=0.009).Conclusion It is useful to measure the Qmax and residual urine in evaluation of bladder function affected by bladder outlet obstruction caused by BPH.
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Objective To evaluate the role of transurethral resection of the prostate(TURP)in early diagnosis of prostate carcinoma.Methods TURP were perforhaed on 16 suspected patients with prostate carcinoma,based on their mean serum PSA level(11.7 ng/ml),negative results of anal touch and needle system biopsies,or with lower urinary tract symptoms,or those biopsy was not fit.6 cases were diagnosed prostate carcinoma by pathology.3 cases underwent double orchieetomy and treated with anti-androgen drugs.2 cases were emasculated by medicines and treated with anti-androgen drugs.1 case underwent radical prostatectomy after neoadjuvant hormonal therapy.Results 6 cases(37.5%)diagnosed prostate carcinoma by pathology were followed up for 3 months to 2 years and were all alive.Lower urinary tract symptoms were relived in 2 patients who underwent TURP. Conclusion TURP play a important role in early diagnosis of prostate carcinoma in certain circumstances.The symptoms could be relieved by TURP in patients with lower urinary tract symptoms.
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Objective To explore the role and significance of free radicle in the pathophsiological mechanisms of diabetic cystopathy(DCP).Methods The detrusor strip contraction experiments were performed and observed in the group of type 2 diabetes mellitus(T2DM) rats at different time stages and the control group.The bladder tissue samples was made into homogenate,and the activities of superoxide dismutase(SOD) and the contents of malondialdehyde(MDA) were determined.Results The minimum stretch forces to induce the detrusor contractions in T2DM group were larger than the control group.Compared with the control group,the detrusor contractive frequency was higher in the period of 0 to 16 weeks,but was lower after the 20th week.The maximum detrusor contractive forces in the T2DM group showed a descended tendency with the elapse of the experiment time.In the bladder homogenate of the control group,the activities of SOD was declined after reaching the peak at the stage of 8th week,and then escalated at the stage of 24th week.As for the T2DM group,the activities of SOD became higher in the 4th week,than descended at the stage of 8th and 24th weeks.The contents of MDA in both groups showed descended tendency.In T2DM group,MDA became even lower than the control group in the 4th week and became higher than the control group in the 8th week.The ratio of SOD/ MDA in T2DM group was lower than the control group.In details,the ratio of SOD/MDA in T2DM group rose significantly in the 4th week,while declined in the 8th week.There was a negative correlation between the maximum detrusor contractive force and detrusor contractive frequency,and a positive correlation between the maximum detrusor contractive force and the contents of MDA.Conclusion The destrusor functions are impaired by Diabetes Mellitus.In the initial stage of DCP,the bladder functions are normal,because the detrusor had a high ability to remove the free radicle.In the progression stage of DCP,the bladder functions become decompensation,because the organism is seriously injured by the free radicle.So the impairment by free radicle is one of important mechanisms of destrusor impairment of diabetic cystopathy.